The efficiency of stimulated emission depletion (STED) microscopy, leads us to a detailed explanation of the light as described with our theory of Elisme (nick name for Électronisme)
« Nanoparticles give super-resolution microscopy a boost
« A new way of beating the diffraction limit in optical microscopy has been unveiled by physicists in Australia and China. The technique makes use of nanoparticles to improve the efficiency of stimulated emission depletion (STED) microscopy, allowing it to be used with lower levels of illumination than previously possible. »
We must first remove some false ideas or meanings of words and phenomena, which we observe in the course of explanations.
– Photon does not exist. Its creation, action and disappearance have never been nor understood nor explained.
– Atoms and molecules are special compounds, for us, only because we can see them (almost) and manipulate them at our « nanometric level », ie 10-9 meters, while the electrons exist and act at the attometric level of 10– 18 meters.
- Light (and images, radio,…) are used by living beings of certain perturbations of the disposition of the electrons of Ether of space, at precise frequencies.
- See explanations of light in chapter IV of the essai. (To click on chapters, in margin on the left)
« STED involves tagging regions of interest in a sample with fluorescent molecules and using a beam of light to cause the molecules to emit light. A second « depletion » beam of light is focused to a doughnut shape in the sample and suppresses the fluorescence everywhere in the focal region – except at the doughnut’s central hole. By scanning the beams jointly over the sample, the spatial distribution of the fluorescent molecules can be determined at resolutions much smaller than the wavelength of the light used.
« The resolution of STED improves as the intensity of the depletion beam increases. However, if the depletion beam is too powerful it will heat up the sample and destroy it – and this puts a practical limit on the resolution that can be achieved.
« Now, Peng Xi, Dayong Jin and colleagues at Macquarie University and several other institutes in Australia and China have got around this problem by using lanthanide-doped upconversion nanoparticles (UCNPs) in place of fluorescent molecules. »
- Technicians know how to make a luminescent material. Could this way leads to better understand on how to use light to collect electricity in PV cells?
- For photoluminescence, it is a light rays that triggers the phenomenon.
- This light is made of free electrons able to combine with free or participating electrons of existing compounds, atoms or molecules, as they might do for PV cells and as they create electric current.
- By their link, compounds are modified or others created, at same time they form new disturbances in Ether electrons, at different frequencies which depend on the quality (luminescence) of the molecules concerned. New space waves with different light frequencies are created, as explained in Chapter III, always with :
- – Modification or creation of gravity (permanent entropization);
- – Same phenomena for gravitational clouds around the compounds;
- – Increased thermic agitation and heat sensation for certain living beings.
The whole of the phenomenon theoretically simple is excessively complicated, especially since chance has a great importance. Many events can create space waves at the same time without mixing their light or other information bearers, as it is explained in Chapter IV of the essay.
This type of explanation for light is valid everywhere in matters, objects and space Ether.
© – Philippe Dardel – 26th, February 2017